発表論文（List of Publication）
44. 小林 直木、西（川崎）晶子、西 毅
新たに同定された赤血球のS1P輸送体MFSD2B
生化学 90, 581-587 (2018)
43. Kobayashi, N., Kawasaki-Nishi, S., Otsuka, M., Hisano, Y., Yamaguchi, A. and Nishi, T.
MFSD2B is a sphingosine 1-phosphate transporter in erythroid cells.
Sci. Rep. 8, 4969 (2018)
42. Kobayashi N. and Nishi, T.
A rapid fluorescence assay for sphingosine 1-phosphate transporter in erythrocytes
Methods Mol Biol. 1697, 73-82 , doi: 10.1007/7651_2017_25 (2018)
41. Kobayashi, N., Otsuka, M., Yamaguchi, A. and Nishi, T.
Fluorescence-based measurement of sphingosine 1-phosphate transport activity in erythrocyte.
J. Lipid Res. 57, 2088-2094 (2016)
40. 西 毅、久野 悠、小林 直木、山口 明人
新しい創薬標的としての脂質メディエーター分泌輸送体
39. Hisano Y., Nishi T. and Kawahara A.
Sphingosine 1-phosphate signaling via transporters in zebrafish and mice.
“Bioactive Lipid Mediators: Current Reviews and Protocols”,
Ed. Yokomizo T., Murakami, M., Springer. (2015)
38. Nishi T., Kobayashi N., Hisano Y., Kawahara A. and Yamaguchi A.
Molecular and physiological function of sphingosine 1-phosphate transporters.
Biochim. Biophys. Acta -Molecular and Cell Biology of Lipids 1831, 759-765 (2014)
37. Kawahara A. and Nishi T.
Functional and physiological roles of sphingosine 1-phosphate transporters.
“Lysophospholipid Receptors: Signaling and Biochemistry”,
Ed. Hla T., Spiegel, S., Moolenaar W. and Chun J., John Wiley & Sons, Inc. (2013)
36. Hisano Y., Nishi T. and Kawahara A.
The functional roles of S1P in immunity.
J. Biochem. 152, 305-311 (2012)
35. Hisano, Y., Kobayashi, N., Yamaguchi, A. and Nishi, T.
Mouse SPNS2 functions as a sphingoshine 1-phosphate transporter in vascular endothelial cells.
34. Takahashi, M., Kawamura, A., Kato, N., Nishi, T., Hamachi, I. and Ohkanda, J.
Phosphopeptide-dependent fluorescence labeling of 14-3-3zeta proteins by fusicoccin-based
fluorescent probes.
Angew. Chem. Int. Ed. 51, 509-512 (2012)
33. Hisano, Y., Kobayashi, N., Kawahara, A., Yamaguchi, A. and Nishi, T.
The sphingosine 1-phosphate transporter, Spns2 function as an exporter for the phosphorylated form of
immunomodulating agent FTY720 from the cells.
J. Biol. Chem 286, 1758-1766 (2011)
32. Ye, D., Meurs, I., Ohigashi, M., Calpe-Berdiel, L., Habets, K.L., Zhao, Y., Kubo, Y., Yamaguchi, A.,
Van Berkel, T.J., Nishi, T. and Van Eck, M.
Macrophage ABCA5 deficiency influences cellular cholesterol efflux and increases susceptibility to
atherosclerosis in female LDLr knockout mice.
Biochem Biophys Res Commun. 395, 387-394 (2010)
31. Kobayashi, N., Kobayashi, N. Yamaguchi, A. and Nishi, T.
Enzymatic analysis of the sphingosine 1-phospate transport in the rat erythrocyte
J. Biol. Chem 284, 21192-21200 (2009)
30. Kawahara A. Nishi, T. Hisano Y. Fukui H. Yamaguchi A. Mochizuki N.
The Sphingolipid transporter spns2 functions in migration of zebrafish myocardial precursors.
29. 川原 敦雄、 西 毅、山口 明人、望月 直樹
スフィンゴシン-1-リン酸(S1P)の輸送体であるSpns2は心臓前駆細胞の移動を制御する
細胞工学 28, 390-391 (2009)
28. Kawasaki-Nishi S. Yamaguchi, A., Forgac M. and Nishi, T.
Tissue specific expression of the splicing variants of the mouse vacuolar
proton-translocating ATPase a4 subunit.
Biochem. Biophys. Res. Commun. 364, 1032-1036 (2007)
27. Kobayashi, N., Nishi, T., Hirata, T., Kihara, A., Sano, T., Igarashi, Y. and Yamaguchi, A.
Sphingosine 1-phosphate is released from the cytosol of rat platelets with carrier-mediated manner.
J. Lipid. Res. 47, 614-621 (2006)
26. Kubo, Y., Sekiya, S., Ohigashi, M., Takenaka, C., Tamura, K., Nada, S., Nishi, T., Yamamoto, A.,
and Yamaguchi, A.
ABCA5 resides in lysosomes and ABCA5 knockout mice develop lysosomal disease-like symptoms.
Mol. Cell. Biol. 25, 4138-4149 (2005)
25. 西 毅
サブユニットアイソフォームによるV-ATPaseの局在と活性の制御
24. 横山 謙、 西 毅
精巧な仕組みを持つプロトンポンプ、V 型 ATPase
蛋白質 核酸 酵素 49, 2035-2045 (2004)
23. Nishi, T., Kawasaki-Nishi S. and Forgac M.
Expression and function of the mouse V-ATPase d subunit isoforms.
J. Biol. Chem. 278, 46396-46402 (2003)
22. Kawasaki-Nishi S., Nishi, T., and Forgac M.
Interacting helical surfaces of the transmembrane segments of subunits a and c’ of the
yeast V-ATPase defined by disulfide-mediated cross-linking.
J. Biol. Chem. 278, 41908-41913 (2003)
21. Shao E., Nishi, T.., Kawasaki-Nishi S. and Forgac M.
Mutational analysis of the non-homologus region of subunit A of the yeast V-ATPase
J. Biol. Chem. 278, 12985-12991. (2003)
20. Nishi, T., Kawasaki-Nishi S. and Forgac M.
The first putative transmembrane segment of subunit c” (Vma16p) of the yeast
V-ATPase is not necessary for function.
J. Biol. Chem. 278, 5821-5827. (2003)
19. Kawasaki-Nishi S., Nishi, T. and Forgac M.
Proton translocation driven by ATP hydrolysis in V-ATPases.
18. Nishi, T. and Forgac M.
The vacuolar (H+)-ATPase—Nature’s most versatile proton pumps.
Nature Reviews Mol. Cell Biol. 3, 94-103. (2002)
17. Arata Y., Nishi, T., Kawasaki-Nishi S., Shao E., Wilkens S. and Forgac M.
Structure, subunit function and regulation of the coated vesicle and yeast vacuolar (H+)-ATPase.
Biochim. Biophys. Acta – Bioenergetics.1555, 71-74. (2002)
16. Kawasaki-Nishi S., Bowers K., Nishi, T., Forgac M. and Stevens T. H.
The amino-terminal domain of the vacuolar proton-translocating ATPase a subunit controls targeting
and in vivo dissociation and the carboxyl-terminal domain affects coupling of proton transport and
ATP hydrolysis.
J. Biol. Chem. 276, 47411-47420. (2001)
15. Kawasaki-Nishi S.¶, Nishi, T. ¶ and Forgac M. (¶Co-first author)
Arg-735 of the 100 kDa subunit a of the yeast V-ATPase is essential for proton transport.
Proc. Natl. Acad. Sci. USA. 98 12397-12402 (2001)
14. Nishi, T., Kawasaki-Nishi S. and Forgac M.
Expression and localization of the mouse homologue of the yeast V-ATPase 21 kDa subunit c” (Vma16p).
J. Biol. Chem. 276, 34122-34130. (2001)
13. Kawasaki-Nishi S.¶, Nishi, T. ¶ and Forgac M. (¶Co-first author)
Yeast V-ATPase complexes containing different isoform of the 100 kDa a subunit
differ in coupling efficiency and in vivo dissociation.
J. Biol. Chem. 276, 17941-17948. (2001)
12. Nishi, T. and Forgac M.
Molecular cloning and expression of three isoforms of the 100kDa a subunit of the mouse vacuolar
proton translocating ATPase.
J. Biol. Chem. 275, 6824-6830. (2000)
11. Leng X. -H., Nishi, T. and Forgac M.
Transmembrane topography of the 100 kDa a subunit (Vph1p) of the yeast V-ATPase.
J. Biol. Chem. 274, 14655-14661. (1999)
10. Murakami H., Sun-Wada G. -H., Matsumoto M., Nishi, T., Wada Y. and Futai M.,
Human histamine H2 receptor gene: Multiple transcription initiation and tissue-specific expression.
FEBS Lett. 451, 327-331. (1999)
9. Yamada H., Yatsushiro S., Hayashi M., Ishio S., Nishi, T., Yamamoto A., Futai M., Yamaguchi A.
and Moriyama Y.
Metabotropic glutamate receptors negatively regulate melatonin synthesis in rat pinealocytes.
J. Neurosci. 18, 2056-2062. (1998)
8. Yamada H., Yatsushiro S., Hayashi M., Yamamoto A., Nishi, T., Futai M. Yamaguchi A., and Moriyama Y.
Functional expression of a GLT-1 type Na+-dependent glutamate transporter in rat pinealocytes.
J. Neurochem. 69, 1491-1498. (1997)
7. Nishi, T., Kubo K., Hasebe M., Maeda M. and Futai M.
Transcriptional activation of H+/K+-ATPase genes by gastric GATA binding proteins.
J. Biochem. 121, 922-929. (1997)
6. 西 毅、二井 將光
胃粘膜壁細胞のプロトンポンプとその発現調節
臨床科学 33, 611-617 (1997)
5. Maeda M., Kubo K., Nishi, T. and Futai M.
Roles of gastric GATA DNA-binding proteins.
J. Exp Biol. 199, 513-520. (1996)
4. Maeda M., Asahara S., Nishi, T., Mushiake S., Oka T., Shimada A. Chiba T., Tohyama M. and Futai M.
The rat intrinsic factor gene: its 5'-upstream region and chief cell-specific transcription.
J. Biochem. 117, 1305-1311. (1995)
3. Nishi, T., Koike T., Hasebe, M. Oka T., Maeda M. and Futai M.
Identification of the promoter region of the human histamine H2-receptor gene.
Biochem Biophys Res Commun. 210, 616-623. (1995)
2. Tamura S., Oshiman K., Nishi, T., Mori M., Maeda M. and Futai M.
Sequence motif in control regions of the H+/K+ ATPase alpha and beta subunit genes
recognized by gastric specific nuclear protein(s).
FEBS Lett. 298, 137-141. (1992)
1. Kobayashi K., Komatsu T., Nishi, T., Hara Y. and Hayashi K.
ATP-dependent transport for glucuronides in canalicular plasma membrane vesicles.
Biochem Biophys Res Commun. 176, 622-626. (1991)
